The Recombinant Enterokinase Activity Detection Kit is a sensitive and reliable enzymatic assay designed to detect and quantify the activity of recombinant enterokinase (enteropeptidase). Enterokinase is a highly specific serine protease that recognizes and cleaves substrates containing the Asp-Asp-Asp-Asp-Lys (DDDDK) recognition sequence, commonly used in recombinant protein processing.
Assay Principle
This kit utilizes a synthetic enterokinase-specific substrate that releases a measurable signal upon enzymatic cleavage. The generated signal—fluorescent or colorimetric depending on the assay format is directly proportional to enterokinase activity in the tested sample, enabling accurate and quantitative analysis.
Key Applications
Measurement of recombinant enterokinase activity
Monitoring enzymatic cleavage efficiency in protein expression workflows
Quality control of enzyme preparations
Biochemical and protease activity studies
High sensitivity and specificity for enterokinase
Fast and reproducible results
Compatible with standard microplate readers
Suitable for low- and high-throughput screening
Ready-to-use reagents with straightforward workflow
Kit Components
The kit includes optimized assay buffers, enterokinase-specific substrate, and control reagents necessary for reliable activity measurement.
Research Use
For research use only. Not intended for diagnostic or therapeutic applications.
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diagnostic kits, antibodies, ELISA solutions, and scientific instruments
trusted by researchers worldwide.